6+ January - February 2015
Acaricidal and antibacterial activities of Xenorhabdus stockiae on
different culture conditions
Chirayu Sa-uth*, Paweena Rattanasena, Prapassorn Bussaman and Angsumarn Chandrapatya
Ph.D. candidate, Lecturer, Asst. Prof., Biological Control Research Unit, Department of Biotechnology, Faculty of Technology,
Mahasarakham University, Maha Sarakham, 44150 Thailand.
Prof., Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
* Correspondent author: email@example.com
Xenorhabdus stockiae is an obligate symbiont bacterium of the entomopathogenic nematode, Steinernema
siamkayai Stock, Somsook and Reid, and it has been previously shown to have acaricidal and antibacterial activities.
In this study, different media were evaluated for enhancing the acaricidal and antibacterial activities of X. stockiae.
Luria Bertani broth (LB), tryptone soyptone broth (TSB) and modified yeast extract broth (YSG) were used for growing
X. stockiae for 24, 48 and 72 h. The maximum acaricidal activities against Luciaphorus perniciosus Rack, mushroom
mite, were found after application with cell-free supernatant ofX. stockiae grown on TSB medium for 48 h (88.50?1.00%)
and 72 h (86.00?3.26%) followed by cell suspension grown on TSB for 48 h (85.00?2.00%). The surviving female mites
were found to produce significantly less number of offspring, accounting for 113.50?10.15, 120.50?5.92 and 127.00?7.87
offspring/gravid female respectively, when compared to the control treatment (distilled water) that resulted in
312.75?11.02 offspring/gravid female. The culture supernatant ofX. stockiae grown using TSB medium for 48 h was
shown to have maximum antibacterial activities, particularly against Staphylococcus epidermidis and Klebsiella
pneumoniae. This study suggested that TSB medium was found to be the optimum medium for cultivation of X. stockiae
to enhance its acaricidal and antibacterial activities.
Keywords : Xenorhabdus stockiae, Luciaphorus perniciosus, mushroom mite, acaricidal activity, antibacterial activity